Coding

Part:BBa_K209408:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-09-30)

AarI C-D part, Intersectin (ITSN)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1388
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1388
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1388
    Illegal BglII site found at 1508
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1388
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1388
    Illegal NgoMIV site found at 159
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Cloned into pCR2.1.

Source

References